The pope also denounced “attacks, even targeted ones, and killings” in an address to thousands of pilgrims and tourists in St Peter’s Square.
Such attacks “can never be a solution”, he said, adding that they “do not help us walk in the path of justice, the path of peace, but generate even more hatred and revenge”.
Tension has escalated between Hezbollah and Israel since Tel Aviv assassinated senior military commander Fouad Shukr in an airstrike in Beirut’s southern suburb on Tuesday.
Hamas political chief Ismail Haniyeh was also assassinated in Tehran the following day, in an attack blamed on Israel although Tel Aviv has not confirmed or denied its responsibility.
Hamas and Iran have vowed to retaliate for Haniyeh’s assassination, while Hezbollah has pledged to respond to Shukr’s killing.
Israel, flouting a UN Security Council resolution demanding an immediate cease-fire, has faced international condemnation amid its continued brutal offensive on Gaza since an attack last October by the Palestinian resistance group Hamas.
Nearly 39,600 Palestinians have since been killed, mostly women and children, and nearly 91,400 injured, according to local health authorities.
Almost 10 months into the Israeli war, vast tracts of Gaza lie in ruins amid a crippling blockade of food, clean water, and medicine.
Israel is accused of genocide at the International Court of Justice for its actions in the coastal enclave.